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Elisa HBcAb Test

  • Price:

    Negotiable

  • minimum:

  • Total supply:

  • Delivery term:

    The date of payment from buyers deliver within days

  • seat:

    Beijing

  • Validity to:

    Long-term effective

  • Last update:

    2017-09-07 00:41

  • Browse the number:

    94

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Company Profile

Shanghai Hengyue Medical Instruments Co. Ltd

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Contact: egens-bio(Mr.)  

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Telephone:

Area: Beijing

address: Apt. 807, No. 2 Building, Lane 3088, Fortune Plaza, Gonghexing Road, Shanghai, Shanghai, China (200072)

Website: http://egens-bio.shanggaoo.com/

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Product details
Model Number: ELISA HBcAb Key Specifications/Special Features: Assay procedure:Prepare reagents: dilute 1 volume of concentrated washing buffer (20x) with 19 volumes of distilled water, mix wellAdd samples and conjugate: open the foil pouch and remove the microplateSet up 1 well as blank, 2 wellsas negative control, 2 wells as positive controlAfter dispensing 50LSamples and 50L
  • Negative control or positive control to the respective wells, add 50μl of conjugate into each well except the blank
  • Gently vibrating the plate
  • Incubate: cover the microplate with plate cover and incubate the microplate in a thermostat-controlled water-bath or microplate incubator at 37C for 30 minutes
  • Wash the plate: remove the plate cover, aspirate the contents of all wells, fill the wells with the diluted washing buffer (10-20 seconds to soak) then aspirate again
  • Repeat the procedure for 5 times
  • Make sure that the rest volume is minimal, by tapping plate onto absorbent paper
  • Add substrate: add 50L of substrate solution A and 50L of substrate solution B to each well, mix well
  • Cover and incubate at 37C for 10 minutes
  • Stop reaction: add 50L stop solution to each well, mix well
  • Read the absorbance at 450nm
  • If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm
  • Results:
    • For the assay to be valid, the mean OD value of positive controls must be less than or equal to 0.1 and the mean OD value of negative controls must be greater than or equal to 0.8
    • Cut off value: PCx0.6+NCx0.4
    • PC: mean absorbance value for two positive controls
    • NC: mean absorbance value for two negative controls
    • Important: if the NC is higher than 1.5, take it as 1.5
    • OD value of the sample ≥ cut off value, it is negative for HBcAb
    • OD value of the sample < cut off value, it is positive for HBcAb
  • Precautions:
    • Allow all kit components to reach room temperature before use
    • Follow the direction insert to control the reaction temperature and time strictly
    • Do not mix components of different lot numbers to use
    • Kit should be store at 2-8C
    • Do not use kit components beyond their expiration date
    • Shipping Information:
    • FOB Port: Shanghai
    • Lead Time: 20 - 25 days
    Main Export Markets:
    • Asia
    • Australasia
    • Central/South America
    • Eastern Europe
    • Mid East/Africa
    • North America
    • Western Europe